Fig. 5

Functional assay of OsNRAMP2 by heterologous expression in yeast. Yeast INVSc1 strain and Δycf1 mutant strain expressing the empty vector (pYES2), OsNRAMP2-H and OsNRAMP2-L were grown in SD-U medium containing concentrations of 0 and 5 μM/10 μM Cd in the presence of glucose (a, b) to suppress the expression of transformed gene, or galactose (c, d) to induce the expression of transformed gene. E, Cd accumulation in Δycf1 strain expressing empty vector and OsNRAMP2-H, OsNRAMP2-L after exposure to 5 μM Cd for 1, 2 and 4 h. Data are means ± SD of three biological replicates. *** p < 0.001 in t-test