Fig. 6
From: A semi-dominant mutation in a CC-NB-LRR-type protein leads to a short-root phenotype in rice
Staining for dead cells, NO and ROS in nrtp1-D mutant roots. 7-d-old seedling root tips were stained by (a) Evans blue (to stain dead cells), (b) 4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate (to detect NO levels), (c) 3′-(ρ-hydroxyphenyl) fluorescein (to detect ROS levels) and (d) Nitro blue tetrazolium (to detect H2O2 and O2.- levels), respectively. Insertion with red frame, positive control. Bars = 100 μm