Fig. 2
From: A semi-dominant mutation in a CC-NB-LRR-type protein leads to a short-root phenotype in rice
Map-based cloning of NRTP1. (a) Physical map of the NRTP1 locus. The number of recombinants per 1500 meioses is indicated for each molecular marker represented by blue bars. N04, I18, E08 and E17 are abbreviations of BAC clones corresponding to OSJNBa0094N04, OSJNBa0056I18, OSJNBb0071E08 and OSJNBb0112E17, respectively. For the structure of the NRTP1 gene, two boxes indicate the two exons and the line indicates the intron. (b) Structure of the NRTP1 protein. Arrow indicates the substitution in the 426-amino-acid protein in the nrtp1-D mutant. (c) A derived cleaved amplified polymorphic sequences (dCAPS) marker differentiates the wild type, heterozygous and homozygous mutant. (d) Complementation test. KASnrtp1-D, two independent transgenic lines harboring mutated DNA fragmentexhibited the heterozygous nrtp1-D mutant phenotype. KAS, wild-type control. Insertion is the dCAPS marker confirmation of transgenic lines. Bar = 2 cm