Fig. 8

Investigating growth responses, survival rate, and endogenous ABA contents in both WT and Osbhlh035 seedlings after recovery from salt stress. a-b The growth responses of aerial and terrestrial tissues in both WT and Osbhlh035 seedlings after relief from salt stress. c The primary root lengths of WT and Osbhlh035 seedlings after relief from salt stress. d The survival rate of WT and Osbhlh035 seedlings after relief from salt stress. All survival rates are shown as the mean ± SD of two independent experiments (n = 20 × 3 independent biological replicates within each genotype and/or experimental condition). e The endogenous ABA contents of WT and Osbhlh035 seedlings after recovery from salt stress. Scale bars in (a) and (b) represent 4 cm. The seedlings were grown on basal medium for 7 days and then transferred to basal medium containing 0 (control) or 100 mM NaCl (recovery) for an additional 7 days. After day 14, the seedlings were returned to basal medium for 7 days. Samples for the ABA ELISA were harvested on day 21. Asterisks indicate significant differences in comparison to WT (*P < 0.05 and **P < 0.01) based on Student’s t-test