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Fig. 3 | Rice

Fig. 3

From: The transcription factor OsbHLH035 mediates seed germination and enables seedling recovery from salt stress through ABA-dependent and ABA-independent pathways, respectively

Fig. 3

The delayed-germination phenotype of Osbhlh035 mutants is accompanied by the excess accumulation of endogenous ABA. a The germination of WT and Osbhlh035 seeds under normal and salt-treated conditions at 2 and 5 days after imbibition. Arabic numerals represent three independent biological replicates, and the seeds in each independent biological replicate were harvested from an individual rice plant. b The germination rates of WT and Osbhlh035 seeds under normal and salt-treated conditions. All germination rates represent the mean ± SD of two independent experiments performed with three independent biological replicates; 100 seeds were used in each independent biological replicate. c The endogenous ABA contents in both germinating WT and Osbhlh035 seeds grown under normal and salt-treated conditions on day 5. d The delayed-germination phenotype of Osbhlh035 mutants can be rescued by fluridone. The germination rates of WT and Osbhlh035 seeds under normal conditions at day 2 (upper panel) and salt-treated conditions at day 5 (lower panel). The seeds were grown on basal medium containing 0 (normal conditions, CK) or 250 mM NaCl (salt-treated conditions) supplemented with or without 50 μM fluridone. Samples for the ABA ELISA were harvested on day 5. Asterisks indicate significant differences in comparison with WT (*P < 0.05 and **P < 0.01) based on Student’s t-test

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