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Rice
Fig. 4 | Rice

Fig. 4

From: The rice white green leaf 2 gene causes defects in chloroplast development and affects the plastid ribosomal protein S9

Fig. 4

Complementation and targeted deletion experiments. a From left to right: wild type (WT), wgl2 with pCAMBIA1300 (pCK/wgl2), and seedlings from three independent WGL2 complementation lines (COM-1, 2, and 3) (bar = 1 cm). b Relative WGL2 transcript levels in WT, wgl2, and complementation transgenic plants. c Sequence confirmation of the WGL2 deletion in WT plants harboring Cas9/sgRNA constructs. The 20-nt target sequence of the Cas9/sgRNA complex is underlined in blue, and the PAM site is indicated in green. For the Cas9/sgRNA-mutated DNA sequences, inserted nucleotides are shown in red, and deleted nucleotides are depicted as yellow dots. d Phenotypes of mutant seedlings harboring the WLG2 Cas9/sgRNA constructs (bar = 1 cm). e Relative transcript levels of WGL2 in WT and three independent mutant transgenic plants. The WGL2 transcript levels in WT were set to 1. Values represent the mean ± SD of 3 biological replicates

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