Fig. 7

OsSND2 and R2R3-MYBs regulates its target genes expression. a Relative expression of R2R3-MYBs genes in WT, OsSND2-OX and snd2-c1 mutant lines. The Actin1 gene was used as internal control. Error bars, SD of three biological replicates. b OsSND2 directly binds to OsMYB61L and OsMYB86L promoters. Yeast one-hybrid assay showing the activity of LacZ reporters driven by OsMYB58.63, OsMYB61L, OsMYB86L, OsMYB103L promoters and activated by activation domain (AD) fusion effectors. The empty pB42AD and pLacZi were used as negative control. c OsSND2 activates transcription of the OsMYB61L and OsMYB86L gene promoter. Luciferase activities in rice protoplasts contransfected with the effectors and reporters. The transactivation activity was monitored by assaying the luciferase activities, with the activity in protoplasts transfected with an empty effector construct defined as 1. Error bars, SD of three biological replicates. d Diagram of OsCESA4 showing three SMRE elements within the 600 bp OsCESA4 promoter region. Black arrows indicate the SMRE elements. The DNA sequences containing a core motif of SMREs (the red bases). The DNA sequence containing these three SMREs was subjected to the EMSA assay. e Yeast one-hybrid assay showing the activity of LacZ reporters driven by OsCESA4 promoter containing three SMRE sites and activated by activation domain (AD) fusion effectors. The empty pB42AD and pLacZi were used as negative control. f OsMYB61L and OsMYB86L activate transcription of the OsCESA4, OsCESA7 and OsCESA9 genes promoter. Luciferase activities in rice protoplasts contransfected with the effectors and reporters. The transactivation activity was monitored by assaying the luciferase activities, with the activity in protoplasts transfected with an empty effector construct defined as 1. Error bars, SD of three biological replicates