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Table 6 Comparison of genotyping cost (USD) considering advancement of all QTL classes versus advancement of only higher performing F3 generation QTL classes

From: Marker-assisted selection strategy to pyramid two or more QTLs for quantitative trait-grain yield under drought

Background

Generation

Number of QTL classes

Population size

Cost (USD)

Total genotyping cost (USD)

Savings (USD)

   

Based on all classes

Based on selected classes

Based on all classes

Based on selected classes

Based on all classes

Based on selected classes

 

Swarna-Sub1

F3

15

754

754

5655

5655

21,420

8978

12,443

F4

15

754

106

5655

795

F5

10

432

106

3240

795

F6

10

432

106

3240

795

F7

6

432

108

3240

810

F8

5

52

17

390

127.50

IR64-Sub1

F3

20

467

467

7005

7005

12,105

8385

3720

F4

19

194

46

2910

690

F5

19

64

18

960

270

F6

13

64

18

960

270

F7

7

18

10

270

150

Samba Mahsuri

BC1F3

3

42

42

210

210

21,760

6980

14,780

BC1F4

3

3000

640

15,000

3200

BC1F5

3

1200

640

6000

3200

BC1F6

3

70

44

350

220

BC1F7

2

20

15

100

75

BC1F8

2

20

15

100

75

TDK1-Sub1

BC2F3

14

843

843

6323

6323

9225

6954

2272

BC2F4

7

231

43

1733

323

BC2F5

7

48

14

360

105

BC2F6

7

48

14

360

105

BC2F7

5

60

13

450

98

MR219

BC1F3

7

214

214

1605

1605

11,955

5730

6225

BC1F4

7

620

240

4650

1800

BC1F5

7

620

240

4650

1800

BC1F6

7

70

35

525

262.50

BC1F7

7

70

35

525

262.50

  1. The genotyping cost was calculated considering five markers per QTL (one peak/near the peak, two right-hand-side flanking markers, and two left-hand-side flanking markers) and USD 0.50 per data point