Fig. 5
From: Outgrowth of Rice Tillers Requires Availability of Glutamine in the Basal Portions of Shoots
qPCR analysis of OsASs in the basal portions of wild-type shoots using MSX. Wild-type rice seedlings were grown hydroponically in the presence of 1 mM NH4Cl until the fourth-leaf stage and then transferred into water for 3 d. After pre-treatment with 1 mM MSX for 2 h, the seedlings were treated for 8 h with 1 mM NH4+ (MSX + NH4+) or 5 mM glutamine (MSX + Gln). The seedlings without MSX pre-treatment were also treated for 8 h with 1 mM NH4+ (+NH4+), 5 mM glutamine (Gln) or without nitrogen nutrients (-N). qPCR analyses of OsAS1 (a), OsAS2 (b), and actin1 transcripts (c) were performed in the shoot basal parts. Fold changes of transcript levels in each treatment relative to those in –N were calculated, and mean values with SE of four independent samples are shown. Asterisks denote statistically significant differences between the samples treated without nitrogen nutrients (-N) and each treated sample (*, P < 0.05 by Student’s t-test)