Fig. 4
From: Outgrowth of Rice Tillers Requires Availability of Glutamine in the Basal Portions of Shoots
Expression levels of OsASs and contents of asparagine and NH4+ in the basal portions. a - c Transcript contents of the OsAS1 (a), OsAS2 (b) and a control Actin1 (c) in the basal portions of shoots of wild-type plants (WT: black column) and gs1;2 mutants (gs1;2: grey column) at the seventh-leaf stage for seedlings grown in water for 3 d followed by treatments with (+NH4+) or without (-N) 1 mM NH4Cl for 8 h. (d, e) Contents of asparagine (d) and NH4+ (e) in the basal portions of shoots of WT (black column) and gs1;2 (grey column) at the fourth-leaf stage for seedlings grown in water for 3 d followed by treatments with or without 1 mM NH4Cl for 24 h. Mean values with SE of four independent samples are shown. Asterisks denote statistically significant differences between samples treated with and without 1 mM NH4Cl (*, P < 0.05 by Student’s t-test)