Fig. 1

qPCR analysis of OsASs in the basal portions of wild-type and gs1;2 mutant shoots. qPCR analysis of OsAS1 and OsAS2 genes (a) and a control Actin1 (b) in the basal portions of shoots of wild-type plants (WT: black column) and gs1;2 mutants (gs1;2: grey column) were performed. Rice seedlings were grown hydroponically in the presence of 1 mM NH₄Cl until the fourth-leaf stage. Mean values with SE of four independent samples are shown. An asterisk denotes a statistically significant difference between wild type and gs1;2 mutants (*, P < 0.05 by Student’s t-test)