Fig. 5

Effects of salt stress on the dynamics of brefeldin A (BFA) compartment labelling by OsPIP2;5 constructs in rice root cells. Root systems of OsPIP2;5-GFP expressing line were incubated in either water (mock condition; open bars) or 100 mM NaCl (closed bars), both supplemented with 50 μM BFA. The number of cells with at least one labelled BFA compartment was counted from images acquired by confocal microscopy, from time 0 to the indicated times. This number was then normalized to the total cell number observed of each root type (crown and lateral roots). Numbers in parentheses indicate the number of cells observed in each assay. The mean values and ± SEs are indicated