Fig. 1

Characterization and phenotypic analysis of the mutants (KO). a OsINV3 gene in 5′-3' direction with the Tos17 insertion in the second exon in reverse orientation. Grey boxes denote exons separated by lines that denote introns, white boxes indicate 5′(left) and 3′(right) UTR, and arrows indicate primers F1, R1 and T5, b The construct used for complementation test containing the ORF of OsINV3 along with 2212 bp upstream of the transcription start site, grey boxes indicating the exons, and arrows indicate primers INV3-PL2 and INV3-R7T, c Gel data showing the presence of WT and KO alleles in WT, KO and complement lines, d mRNA transcript level of OsINV3 relative to RUBIQ (n = 3) and e Vacuolar invertase activity expressed as Vmax (n = 5), in mature leaf of WT, KO and C4, f Panicles from WT, KO and C4 lines, g Grain length and width parameters for WT, KO and C4 (n = 12), h Spikelet area for filled and unfilled spikelets from WT, KO and C4 lines (n = 12), i average weight per grain, for WT, KO and C4 lines (n = whole grain set from 3 plants). Data represent the mean ± SE. Same letters indicate insignificant differences between the lines tested using ANOVA (Tukey’s test) with significance level of p < 0.05. Vertical white bars indicate 1 cm