Fig. 7
From: Rice WRKY11 Plays a Role in Pathogen Defense and Drought Tolerance
Degradation of OsWRKY11 protein by the UPS. a Rice (Oryza sativa) protoplasts were transformed with 35S::OsWRKY11-YFP. After 4 h, protoplasts were treated with 100 μM MG132 and buffer for 1 day. Fluorescence was observed using a confocal laser scanning microscope. Scale bars: 30 μm. NT: non-treated control. b Immunoblot analysis of total protoplast proteins in the absence (−) or presence (+) of 100 μM MG132. Blots were probed with green fluorescent protein (GFP) antibody. The PAT antibody was used as a control for transformation efficiency