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Fig. 4 | Rice

Fig. 4

From: Rice WRKY11 Plays a Role in Pathogen Defense and Drought Tolerance

Fig. 4

OsWRKY11 directly binds to and activates the CHITINASE 2 promoter. a Nicotiana benthamiana leaf discs were infiltrated with Agrobacterium carrying pCHIT2::GFP-GUS alone or with a mixture of Agrobacterium carrying 35S::OsWRKY11 and pCHIT2::GFP-GUS. Promoter activities in each sample were visualized 2 days post-infiltration using β-glucuronidase (GUS) activity staining. b Transgenic rice leaves expressing 35S::HA-OsWRKY11 were used for chromatin immunoprecipitation with anti-HA antibody. ChIP-PCRs were performed on genomic DNA fragments using promoter-specific primers against CHIT2 (Table S1). To normalize qPCR values, the value obtained from the sample with pre-immune serum (no antibody) was divided by the value obtained from the sample with 10% input of each sheared chromatin sample and arbitrarily set at 1. The value resulting from OsWRKY11 binding to the promoter was expressed as a relative ratio of the pre-immune serum sample. Asterisks indicate significant differences between treatments from the no-antibody control sample (**: P < 0.01; *: P < 0.05)

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