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Fig. 4 | Rice

Fig. 4

From: Physiological and molecular responses of seedlings of an upland rice (‘Tung Lu 3’) to total submergence compared to those of a submergence-tolerant lowland rice (‘FR13A’)

Fig. 4

Activities of antioxidative enzymes in ‘TL3’ and ‘FR13A’ rice seedlings under submerged conditions. a 3,3′-diaminobenzidine (DAB) staining of H2O2 in detached 2nd oldest leaves (L2) and 3rd oldest leaves (L3) of 10-d-old rice seedlings after 6 or 10 d in air (control) or full submergence (Sub). Similar results were obtained in three independent experiments. Photographs show the results of three independent leaves. Bar = 3 cm. b Enzyme activity of detached 2nd oldest leaves (L2) and 3rd oldest leaves (L3) of 10-d-old rice seedlings after 2d in air (control) or full submergence (Sub) and then allowed to recover for 1 d (Re). Ascorbate peroxidase (APX), catalase (CAT), total peroxidase (POX) and superoxide dismutase (SOD) activities are average values ± standard deviation of four biologically independent experiments each with duplicate samples. Values with the different letters are significantly different at P < 0.05, according to a post-hoc LSD test

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