Fig. 5

Silencing of XB21 in Kitaake plants does not affect resistance to Xoo. a Relative expression of XB21 in Kitaake plants silencing XB21. qRT-PCR was performed with specific primers for each genes. Gene expression level was normalized using ubiquitin as an internal reference. b Lesion lengths measured 14 days after inoculation from Kitaake plants silencing XB21 (XB21RNAi/Kit 9B, 12A, 13A, and 14A) and Kitaake plants. The error bars represent standard error between three biological replicates. c Xoo populations were monitored over 12 days in XB21RNAi/Kit (9B, 12A-2, and 14A-2) and Kitaake wild type plants. For each time point, bacterial populations were obtained in three separate leaves for each genotype. The error bars represent standard deviation values obtained from the three samples