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Rice
Fig. 4 | Rice

Fig. 4

From: Genome-wide identification of grain filling genes regulated by the OsSMF1 transcription factor in rice

Fig. 4

Production of DsRed-fused OsSMF1-transformed rice. a Schematic representation of the expression vector Wsi:SMF1 used for rice transformation. Wsi pro, rice responsive to ABA protein promoter; PinII ter, potato proteinase inhibitor II terminator; 35S pro, cauliflower mosaic virus 35S promoter; Bar, phosphinothricin acetyltransferase; and Nos ter, nopaline synthesis terminator. b The expression of OsSMF1 and its target genes was determined by RT-PCR analysis. Specifically, the expression of granule-bound starch synthase (GBSS) and 26-kDa globulin (α-Glb) were analyzed. Total RNA was extracted from calli. Tubulin expression is shown as a constitutive control

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