Development and Genetic Characterization of A Novel Herbicide (Imazethapyr) Tolerant Mutant in Rice (Oryza sativa L.)

Rice

Table 5 Functional motifs predicted in ALS proteins of WT and HTM-N22

S. No.	Motif sites	WT		HTM-N22
S. No.	Motif sites	Position of the amino acid	Features	Position of the amino acid	Features
1	PS00005 PKC_PHOSPHO_SITE Protein kinase C phosphorylation site	17 – 19	TaK	17 – 19	TaK
		20 – 22	TgR	20 – 22	TgR
		132 – 134	SgR	132 – 134	SgR
		218 – 220	SgR	218 – 220	SgR
		273 – 275	SrR	273 – 275	SrR
		319 – 321	SlR	319 – 321	SlR
		353 – 355	TgK	353 – 355	TgK
		404 – 406	TtK	404 – 406	TtK
		471 – 473	TyK	471 – 473	TyK
		590 – 592	TkK	590 – 592	TkK
2	PS00009 AMIDATION Amidation site	20 – 23	tGRK	20 – 23	tGRK
3	PS00008 MYRISTYL N-myristoylation site	136 – 141	GVcvAT	136 – 141	GVcvAT
		152 – 157	GLadAL
		281 – 286	GGgcSA	281 – 286	GGgcSA
		282 – 287	GGcsAS	282 – 287	GGcsAS
		283 – 288	GCsaSG	283 – 288	GCsaSG
		299 – 304	GIpvTT	299 – 304	GIpvTT
		310 – 315	GNfpSD	310 – 315	GNfpSD
		324 – 329	GMhgTV	324 – 329	GMhgTV
		327 – 332	GTvyAN	327 – 332	GTvyAN
		490 – 495	GLpaAA	490 – 495	GLpaAA
		496 – 501	GAsvAN	496 – 501	GAsvAN
		563 – 568	GNpeCE	563 – 568	GNpeCE
4	PS00006 CK2_PHOSPHO_SITE Casein kinase II phosphorylation site	287 – 290	SgdE	287 – 290	SgdE
		407 – 410	TssD	407 – 410	TssD
		431 – 434	TfgE	431 – 434	TfgE
		449 – 452	TkgE	449 – 452	TkgE
		505 – 508	TvvD	505 – 508	TvvD