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Fig. 7 | Rice

Fig. 7

From: Proteomic and Glycomic Characterization of Rice Chalky Grains Produced Under Moderate and High-temperature Conditions in Field System

Fig. 7

Changes in the expression of α-amylase isoforms in central opaque area of chalky grains harvested in 2009 and 2010. a Immunoblotting images. Center part of perfect grain and opaque part of chalky grain were subjected to protein, followed by SDS-PAGE and immunoblotting with specific antibodies. C, germinating seeds after 5 days of imbibition; lane 1, perfect grain in 2009; lane 2, chalky grain in 2009; lane 3, perfect grain in 2010; lane 4, chalky grain in 2010. b Quantitation of α-amylase isoforms expression. Amount of each α-amylase isoform in perfect grains was normalized to 1 unit. The perfect proteins of AmyII-5 and AmyII-6 were not detected. Values are represented as mean ± s.d. (n = 3). Histograms with the same letter do not differ significantly (p ≤0.05)

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