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Rice
Fig. 3 | Rice

Fig. 3

From: The Rice TCM5 Gene Encoding a Novel Deg Protease Protein is Essential for Chloroplast Development under High Temperatures

Fig. 3

Genetic analysis and cloning of the TCM5 gene; a, The TCM5 gene was initially located between the RM2474 and RM18692 SSR markers on chromosome 5 using 96 F2 mutant individuals; b, TCM5 was narrowed to a 18.9 kb between InDel marker P1 and CAPS marker P3 and co-segregated with the CAPS marker P2 using 6400 F2 mutant individuals; c, The target region contains two predicted candidate genes (LOC_Os05g34450, LOC_Os05g34460); d, A single nucleotide mutation at codon 194(T→G) in the first exon in LOC_Os05g34460, consisting of eight exons; e, The complemented plants with pCAMBIA1301:CaMV35S:TCM5 (left) and transgenic T0 plants with empty vector pCAMBIA1301(right); f, Transcript levels of TCM5 in WT and tcm5 at the 3-leaf stage grown at 32 and 20 °C, OsActin was used as a control for qPCR

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