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Rice
Fig. 2 | Rice

Fig. 2

From: Development and validation of allele-specific SNP/indel markers for eight yield-enhancing genes using whole-genome sequencing strategy to increase yield potential of rice, Oryza sativa L.

Fig. 2

OsSPL14 markers. a Identification of donor-specific DNA polymorphisms in OsSPL14 gene. The donor-specific SNPs at the promoter and the third exon were represented through DNA sequence alignment among 15 varieties, including Nipponbare, two different OsSPL14 donors (ST12 and Aikawa1), and 12 recipients. The location of 2.6-kb candidate region of WFP (Miura et al. 2010) was depicted. b Agarose gel images analyzed by the SPL14-04SNP marker and the SPL14-12SNP marker, respectively, from the parental lines. Primer combination for each PCR was shown on the gel images. c Application of SPL14-04SNP marker in the intermediate breeding line, with 12 BC2F2 plants derived from PR38012 x ST12 cross. d Application of SPL14-12SNP marker in the intermediate breeding line. Twelve BC2F2 plants derived from IR04A115 x Aikawa1 cross were genotyped

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