Fig. 2

Isolation and sequence analysis of the gene conferring responsible for purple leaf sheath. Coarse mapping of the purple leaf sheath gene between CH0639 and RM276 on chromosome 6. Fine mapping narrows down this region to 160.04 kb between CH0611 and RM253. The LOC_06g10350 locus was annotated as MYB family transcription factor by RGAP and corresponded to Os06g0205100 annotated as transcription factor MYB6 and O. sativa C1, and rice homolog of maize C1 by RAP. Sequence analysis of 3 cultivars, Tainung 72 (TNG72), Nipponbare and Taichung Sen 17 (TCS17). TNG72 has complete sequences translated into 272 amino acids. Nipponbare has 3-bp deletion in the second exon of OsC1, and is translated into 271 amino acids. TNG17 has 10-bp deletion in the third exon of OsC1, and is translated into 207 amino acids. The black lines are conserved domains of OsC1, R2R3 Myb domain. The triangles indicate sites of variation