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Fig. 4 | Rice

Fig. 4

From: Physiological Responses and Expression Profile of NADPH Oxidase in Rice (Oryza Sativa) Seedlings under Different Levels of Submergence

Fig. 4

DAB, NBT and Evans blue staining for the detection of H2O2, O2 and cell death in rice seedlings under different levels of submergence. a Phenotypes of 14 days-old rice seedlings after treated control (CK), partial submergence (PS) and full submergence (FS) for 6 days. b and c, DAB and NBT staining of H2O2 and O2 in detached leaves of 14 days-old rice seedlings after treated control (CK), partial submergence (PS) and full submergence (FS) for 6 days. Same results were obtained in three independent experiments. Photograph shows results of three independent leaves. d and e, Evans blue staining and cell death analysis (see materials and methods for details). Bar = 1 cm. The data represent average values ± SD from five biologically independent experiments. Values with the different letters are significantly different at P < 0.05, according to post-hoc LSD test

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