Fig. 5

Overexpression of OsPYL3 and OsPYL9 confer ABA hypersensitivity during seed germination. a Quantitative RT-PCR analysis of OsPYL3 in overexpression transgenic lines. The expression level of OsPYL3 in control line was set as 1 and the fold change was analyzed via the 2^-△△CT method using the rice ubiquitin gene as an internal control. Values represent the mean ± SD of three biological replicates. b RT-PCR analysis of OsPYL9 in overexpression transgenic lines. Full length OsPYL9 primer was used and rice ubiquitin transcripts were used as control. c Representative photographs of seed germination of OsPYL3 and OsPYL9 overexpression transgenic lines. Seeds of control and transgenic lines were grown on half-strength MS medium containing indicated concentration of ABA for 5 days. Photographs were taken on day 5. (D to I) Germination time course of OsPYL3 (d-f) and OsPYL9 (g-i) overexpression transgenic lines and control in medium without ABA (d and g), 1 μM ABA (E and H) and 3 μM ABA (f and i). Data show the mean ± SD of three replicates. At least 50 seeds per genotype were measured in each replicate