Fig. 3

GA responses were enhanced in the seeds carrying the el1(H143) allele. Assays of α-amylase activity in the seeds of HNIL(M23) and HNIL(H143) (a) and M23 and H143 (b) on plates containing starch. The seeds were incubated on plates containing ABA (10 μM) or GA₃ (1 mM) for 3 days at 28 °C. The numbers of seeds with α-amylase activity were compared by Student’s t-test (***P < 0.001, n = 40). This experiment was repeated at least 3 times. c Length of the epidermal cells in the 2nd leaf sheath of HNIL(M23) and HNIL(H143) plants. The plants were treated with uniconazole (Uni; GA biosynthesis inhibitor) for 1 day and grown in a growth chamber for 7 days. Student’s t-test was used for statistical analysis (***P < 0.001, n = 10)