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Table 3 Number of triple resistant gene heterozygotes identified and estimation of recurrent parent genome contribution

From: Pyramiding of three bacterial blight resistance genes for broad-spectrum resistance in deepwater rice variety, Jalmagna

Generation # of plants
scored a
# of plants that are
triple heterozygotes a, b
Estimated maximum %
contribution of recurrent
parent genome to selected
backcross plant c
Expected % contribution
of recurrent parent genome
to selected backcross plant d
BC1F1 360 14 77.5 77.5
BC2F1 122 9 91.8 91.8
BC3F1 285 14 97 97
  1. aAt each backcross generation, genomic DNA was isolated from derivative lines and genotyping was performed using primers that are tightly linked to BB resistant genes as described in Materials and methods.
  2. bAt each backcross generation, fewer than expected triple heterozygotes were obtained. This is due to the fact that some of the
  3. putative backcross progeny were obtained by inadvertent selfing of Jalmagna (the female parent in these backcrosses).
  4. cAt each backcross generation, genomic DNA was isolated from derivative lines that are triple heterozygotes for BB resistant gene linked markers. Microsatellite markers that are polymorphic between the parental lines were then used, as described in Materials and Methods, to identify the plant with maximum recurrent parent genome contribution.
  5. dAs per Mendelian ratios for independent gene action.