Figure 6

Silencing of XIK1 reduces the basal level of PTI marker genes in ProA-Xa21 plants . The relative expression level of two rice PTI marker genes, OSKS4 and Os04g10010, were determined by qRT-PCR using RNA extracted from rice leaves of six weeks old plants without Xoo inoculation (A) and 2 days post Xoo inoculation (B, C). All data were normalized to the expression of the ubiqutin5 reference gene. The results of one representative experiment were shown. Error bars indicating SD of three technical replicates. Three independent biological experiments were repeated with the similar results. The letters indicate significant differences (P<0.05) for each gene determined by a one-way ANOVA followed by post hoc Tukey HSD analysis.