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Rice
Figure 2 | Rice

Figure 2

From: Phenotypic analyses of rice lse2 and lse3 mutants that exhibit hyperaccumulation of starch in the leaf blades

Figure 2

Microscopic observations of transverse sections of fully developed leaf blades of seedlings. The same tissue samples as shown in Figure 1B–E were used. A–D, light micrographs of periodic acid–Schiff-stained semithin sections. Dark red or purple dots showing accumulation of polysaccharides can be seen in lse1 (B), lse2 (C), and lse3 (D), and not in WT (A). Polysaccharide accumulation was pronounced in mesophyll cells (M) and was also evident in bundle sheath cells surrounding vascular bundles (black arrowheads). Blue arrowheads indicate polysaccharide accumulation within the large vascular bundle in lse3 (D). Scale bars = 100 μm. E–H, TEM of small or medium vascular bundles in wild type (E), lse1 (F), lse2 (G), and lse3 (H). Black arrowheads indicate starch granules in bundle sheath chloroplasts; blue arrowheads Indicate starch granules in vascular parenchyma cells. Scale bars = 2 μm. BS, bundle sheath cell; CC, companion cell; M, mesophyll; P, phloem; SE, sieve element; V, vessel element.

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