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Figure 5 | Rice

Figure 5

From: Rice miR172 induces flowering by suppressing OsIDS1 and SNB, two AP2 genes that negatively regulate expression of Ehd1 and florigens

Figure 5

Phenotypes of miR172d OX plants. (A) Scheme of miR172d OX construct. Spanning region of miR172d was sub-cloned into pGA1611 vector between maize ubiquitin promoter (Pubi) and nopaline synthase terminator (nos T). (B) Northern blot analysis of expression levels of mature miR172 in WT and miR172d OX plants (top). Ethidium bromide-stained ribosomal RNA was used as loading control (bottom). (C) Flowering time for 6 miR172d OX plants compared with WT when grown in greenhouse. Days-to-heading was scored when first panicle bolted. (D-G) Expression levels of Ehd1 (D), Hd3a (E), SNB (F), and OsIDS1 (G) in WT and miR172d OX plants.

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