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Figure 1 | Rice

Figure 1

From: Rice miR172 induces flowering by suppressing OsIDS1 and SNB, two AP2 genes that negatively regulate expression of Ehd1 and florigens

Figure 1

Temporal expression patterns of miR172 and AP2 genes. (A, B) RT-PCR analysis of pri-miR172a (A) and pri-miR172d (B) in WT plants under LD. Y-axis, relative transcript level compared with OsUbi1. Error bars indicate standard deviations; n = 4 or more. Middle regions of fully emerged uppermost leaves were sampled at 30, 40, 45, 50, 55, and 60 DAG. (C) Northern blot analysis of mature miR172 (top). Ethidium bromide-stained ribosomal RNA served as loading control (bottom). Middle regions were used from leaves sampled at 25, 30, 40, 45, 50, and 55 DAG. (D, E) RT-PCR analysis of transcript levels of SNB (E) and OsIDS1 (E) under LD. Middle regions were used from leaves sampled at 27, 30, 32, 35, 38, 44, 51, 55, and 57 DAG. (F) RT-PCR analysis of Ehd1 transcripts under LD, using leaves sampled at 27, 30, 32, 35, 38, 44, 51, 55, 57, 60, 63, 66, and 73 DAG. Expression was monitored at ZT 18 h for miR172 and at ZT 2 h for AP2 genes and Ehd1. Y-axis, relative transcript level compared with OsUbi1. Error bars indicate standard deviations; n = 4 or more.

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