Relative expression of the Mi-crt gene in M. incognita -infested rice O. sativa cv. Nipponbare roots after 6, 10, and 20 dai. Gene expression was measured by reverse transcription-quantitative polymerase chain reaction in plants infested with M. incognita at different time points after treatment. The Mi-crt data were normalized to the Mi-actin data and Mi-crt gene expression in infected-rice samples was further calculated relative to the 6 DAI sample. Data presented are means (± standard error of the mean [SEM]) expression in three independent biological replicates, with 35 plants per condition (n=3, each contained 35 plants pooled).