Table 1 PCR primers used in this study
From: Response of an aspartic protease gene OsAP77 to fungal, bacterial and viral infections in rice
Primer | Nucleotide sequencea |
|---|---|
OsAP77 pro-5′ | 5′-ATGCCCTGCAGG ATGACGCAATCAGTCAGACC-3′ |
OsAP77 pro-3′ | 5′-ATGCTCTAGA AGTATCAACCAGCGATGCTA-3′ |
∆GUS-5′ | 5′-AGCTCTAGACTATCCCGCCGGGAATGGTGA-3′ |
∆GUS-3′ | 5′-ACGAATTCGGTAGCAATTCCCGA-3′ |
CMV-R3-cDNA-F-5′ | 5′-CTGCCTCCTCGGACTTATCC-3′ |
CMV-R3-cDNA-R-3′ | 5′-CTGAAACTAGCACGTTGTGC-3′ |
OsAP-5′ | 5′-CGGAATTCATGGGAAGGCCAGTGGCAAC-3′ |
OsAP-3′ | 5′-CTAGATCTCTAGGAGAGTTTAGTGCAGTC-3′ |
AP77P-5′ | 5′-CTCACCGTTGTAGGTAACTG-3′ |
AP77P-3′ | 5′-AGTGAGCACGAAATAACTG −3′ |
Tos17-5′ | 5′-ATTGTTAGGTTGCAAGTTAGTTAAGA −3′ |
Actin-5′ | 5′-GAGTATGATGAGTCGGGTCCAG-3′ |
Actin-3′ | 5′-ACACCAACAATCCCAAACAGA-3′ |