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Table 1 PCR primers used in this study

From: Response of an aspartic protease gene OsAP77 to fungal, bacterial and viral infections in rice

Primer Nucleotide sequencea
OsAP77 pro-5′ 5′-ATGCCCTGCAGG ATGACGCAATCAGTCAGACC-3′
OsAP77 pro-3′ 5′-ATGCTCTAGA AGTATCAACCAGCGATGCTA-3′
∆GUS-5′ 5′-AGCTCTAGACTATCCCGCCGGGAATGGTGA-3′
∆GUS-3′ 5′-ACGAATTCGGTAGCAATTCCCGA-3′
CMV-R3-cDNA-F-5′ 5′-CTGCCTCCTCGGACTTATCC-3′
CMV-R3-cDNA-R-3′ 5′-CTGAAACTAGCACGTTGTGC-3′
OsAP-5′ 5′-CGGAATTCATGGGAAGGCCAGTGGCAAC-3′
OsAP-3′ 5′-CTAGATCTCTAGGAGAGTTTAGTGCAGTC-3′
AP77P-5′ 5′-CTCACCGTTGTAGGTAACTG-3′
AP77P-3′ 5′-AGTGAGCACGAAATAACTG −3′
Tos17-5′ 5′-ATTGTTAGGTTGCAAGTTAGTTAAGA −3′
Actin-5′ 5′-GAGTATGATGAGTCGGGTCCAG-3′
Actin-3′ 5′-ACACCAACAATCCCAAACAGA-3′
  1. aNucleotides in italic in OsAP77 pro-5′ and OsAP77 pro-3′ indicate Sbf I and Xba I sites, respectively.