The accumulation level of Cucumber mosaic virus RNA in the knockout mutant line of OsAP77 . The young leaves were inoculated with Cucumber mosaic virus. Total RNA was extracted from the inoculated leaves at 7 dpi. Upper panel showed analysis of CMV after infection. The actin gene was used as the standard control to show the normalization of the amount of templates in PCR reactions. Nb, Nipponbare (+/+); 3, 4, 5 and 6, OsAP77 (−/−); 2 and 8, OsAP77 (+/−); M, mock in Nipponbare (+/+).