Expression patterns of OsAP77::GUS after with fungal and bacterial pathogens. (A) M. oryzae (virulent race 001, virMo); (B) M. oryzae (avirulent race 102.0, avrMo); (C) X. oryzae pv. oryzae. (D) Rice blast and bacterial leaf blight disease symptoms (upper panel) and ethanol washed symptoms (lower panel). The detached leaves of T3 plant were wounded by a needle-inoculation with/without droplets of conidia suspension (2×105 conidia ml−1) and X. oryzae pv. oryzae at OD600 of 0.3. Then 48 h later leaves were subjected to GUS staining. Transverse sections of leaves were shown just below the leaf image. Brown portion is the symptoms by blast fungi and leaf blight bacteria, respectively. The area neighboring the symptoms stained blue, which is clearly shown in the leaf images as well as in transverse sections. virMo with M. oryzae; virXoo, with X. oryzae pv. oryzae; mock, a needle inoculation with water (wounded); contl., no treatment.