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Figure 1 | Rice

Figure 1

From: Gene targeting using the Agrobacterium tumefaciens-mediated CRISPR-Cas system in rice

Figure 1

Gene editing in rice using the stably expressed sgRNA:Cas9 system. (a) Diagram of the sgRNA:Cas9 complex targeting the rice BEL gene at the target-1 site. PAM, the protospacer adjacent motif. gRNA, the guide RNA scaffold (Mali et al. [2013]). (b) sgRNA:Cas9-induced rice BEL gene mutations at the target-1 site. Upper: 14 mutants were identified in 90 independent lines transformed the target-1 sgRNA:pSpCas9. Lower: 2 mutants were identified in 96 independent lines transformed the target-1 sgRNA:hSpCas9. The yellow shadow marks the target sequence that is recognized by the crRNA. The deletions, insertions, and nucleotide substitutions are marked in red. (c) The phenotypes of the bel mutants. The leaves were sprayed with 1.5 g/L bentazon for 7 days. Upper: the transgenic plant without the target mutation; lower: the biallelic mutant with the site-specific mutation. Scale bar = 1 cm. (d) sgRNA:Cas9-induced rice BEL gene mutations at the target-2 site. 2 mutants were identified in 94 independent lines transformed the target-2 sgRNA:pSpCas9.

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