RT-PCR pattern of Pb1 in backcrossed progeny between Miyazakimochi and Koshihikari. RT-PCR was used to target two subregions of the Pb1 coding region: (A) from 1 to 1587 bp, and (B) from 1536 to 3891 bp. The primers were designed with reference to Hayashi et al. ([2010b]) and the Pb1 genomic sequence (AB570371). Rice G3PDH (glyceraldehyde-3-phosphate dehydrogenase, AK064960) was used as a control gene (C). RT-PCR was carried out with total RNA extracted from panicles of Tsukinohikari (two individuals, A and B), Koshihikari, Miyazakimochi, and four BC3F2 individuals (BC3F2-1 to −4). Tsukinohikari and Koshihikari were used as positive and negative controls for Pb1 amplification, respectively. M = molecular size marker (1 kb Ladder marker, TAKARA).