Iron biofortification of rice using different transgenic approaches

Rice

Table 2 Approaches of Fe biofortification of rice: multi transgenic approaches

Approach	Introduced genes^a	Rice cultivar	Cultivation	Fold increase in Fe concentration compared to non-transgenic rice^b.	References
			condition
Combination of approaches 1 and 2	OsGlb pro-Pvferritin	Japonica cv. Taipei 309	Hydroponic culture	6 fold (polished seeds)	Wirth et al. 2009
	35S pro-AtNAS1
	OsGlb pro-Afphytase
Combination of approaches 1, 2 and 3	OsGluB1 pro-SoyferH2	Japonica cv. Tsukinohikari	Soil cultivation in greenhouse	6 fold (polished seeds)	Masuda et al. 2012
	OsGlb1 pro-SoyferH2
	OsActin1 pro-HvNAS1		Paddy field	4.4 fold (polished seeds)
	OsSUT1 pro-OsYSL2
	OsGlb1 pro-OsYSL2
	OsGluB1 pro-SoyferH2	Tropical Japonica cv. Paw San Yin (Myanmar High Quality Rice)	Soil cultivation in greenhouse	3.4 fold (polished seeds)	Aung et al. 2013
	OsGlb1 pro-SoyferH2
	OsActin1 pro-HvNAS1
	OsSUT1 pro-OsYSL2
	OsGlb1 pro-OsYSL2
Combination of approaches 1 and 4	OsGluB1 pro-SoyferH2	Japonica cv. Tsukinohikari	Normal soil in greenhouse	4 fold (polished seeds)	Masuda et al. 2013
	OsGlb1 pro-SoyferH2
	HvNAS1, HvNAAT-A,-B and IDS3 genome fragments		Calcareous soil in greenhouse	2.5 fold (polished seeds)

^aThese gene expression cassettes were introduced concomitantly. ^b.The tissue name written in parentheses is the rice tissue where Fe concentration was increased.