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Table 2 Approaches of Fe biofortification of rice: multi transgenic approaches

From: Iron biofortification of rice using different transgenic approaches

Approach Introduced genesa Rice cultivar Cultivation Fold increase in Fe concentration compared to non-transgenic riceb. References
    condition   
Combination of approaches 1 and 2 OsGlb pro-Pvferritin Japonica cv. Taipei 309 Hydroponic culture 6 fold (polished seeds) Wirth et al. 2009
  35S pro-AtNAS1     
  OsGlb pro-Afphytase     
Combination of approaches 1, 2 and 3 OsGluB1 pro-SoyferH2 Japonica cv. Tsukinohikari Soil cultivation in greenhouse 6 fold (polished seeds) Masuda et al. 2012
  OsGlb1 pro-SoyferH2     
  OsActin1 pro-HvNAS1   Paddy field 4.4 fold (polished seeds)  
  OsSUT1 pro-OsYSL2     
  OsGlb1 pro-OsYSL2     
  OsGluB1 pro-SoyferH2 Tropical Japonica cv. Paw San Yin (Myanmar High Quality Rice) Soil cultivation in greenhouse 3.4 fold (polished seeds) Aung et al. 2013
  OsGlb1 pro-SoyferH2     
  OsActin1 pro-HvNAS1     
  OsSUT1 pro-OsYSL2     
  OsGlb1 pro-OsYSL2     
Combination of approaches 1 and 4 OsGluB1 pro-SoyferH2 Japonica cv. Tsukinohikari Normal soil in greenhouse 4 fold (polished seeds) Masuda et al. 2013
  OsGlb1 pro-SoyferH2     
  HvNAS1, HvNAAT-A,-B and IDS3 genome fragments   Calcareous soil in greenhouse 2.5 fold (polished seeds)  
  1. aThese gene expression cassettes were introduced concomitantly. b.The tissue name written in parentheses is the rice tissue where Fe concentration was increased.