From: Iron biofortification of rice using different transgenic approaches
Approach | Introduced genes | Rice cultivar | Cultivation condition | Fold increase in Fe concentration compared to non-transgenic ricea | References |
---|---|---|---|---|---|
Approach 1 | OsGluB1 pro-SoyferH1 | Japonica cv. Kitaake | Soil cultivation in greenhouse | 2 fold (polished seeds) | Goto et al. 1999 |
Enhancement of Fe storage in rice seeds by ferritin | Â | Â | Â | Â | Â |
 |  |  |  | 3 fold (brown seeds) |  |
 | OsGluB1 pro- SoyferH1b | Japonica cv. Kitaake | Soil cultivation in greenhouse | 1.5 fold (brown seeds) | Qu et al. 2005 |
 | OsGlb1 pro-SoyferH1b |  |  |  |  |
 | OsGluB1 pro-SoyferH1 | Japonica cv. Taipei 309 | Soil cultivation in greenhouse | 2.2 fold (brown seeds) | Lucca et al. 2002 |
 | OsGluB1 pro-SoyferH1 | Indica cv. IR68144 | Soil cultivation in greenhouse | 3.7 fold (polished seeds) | Vasconcelos et al. 2003 |
 |  | (High Fe breeder line) |  |  |  |
 | OsGluA2 pro-OsFer2 | Indica cv. Pusa-Sugandh II (Aromatic rice) | Soil cultivation in greenhouse | 2.1 fold (polished seeds) | Paul et al. 2012 |
Approach 2 | OsActin1 pro-HvNAS1c | Japonica cv. Tsukinohikari | Soil cultivation in greenhouse | 2 fold (polished seeds) | Masuda et al. 2009 |
Enhancement of Fe translocation by overexpression of NAS | Â | Â | Â | Â | Â |
 | 35S pro-HvNAS1c |  |  |  |  |
 | Activation tag line of OsNAS3 | Japonica cv. Dongjin | Soil culture in greenhouse | 3 fold (polished seeds) | Lee et al. 2009c |
 | 35S pro- OsNAS1, 2, 3 | Japonica cv. Nipponbare | Soil cultivation in greenhouse | 4 fold (polished seeds) | Johnson et al. 2011 |
Approach 3 | OsSUT1 pro-OsYSL2 | Japonica cv. Tsukinohikari | Soil cultivation in greenhouse | 4 fold (polished seeds) | Ishimaru et al. 2010 |
Enhancement of Fe transportation by Fe transporter | Â | Â | Â | Â | Â |
Approach 4 | Barley IDS3 genome fragment | Japonica cv. Tsukinohikari | Andosol soil in paddy field | 1.4 fold (polished seeds) 1.3 fold (brown seeds) | Masuda et al. 2008 |
Enhancement of Fe uptake and translocation by IDS3 gene | Â | Â | Â | Â | Â |
 |  |  | Calcareous soil in paddy field | 1.3 fold (brown seeds) | Suzuki et al. 2008 |
Approach 5 | Ubiquitin pro-OsIRT1 | Japonica cv. Dongjin | Paddy field | 1.7 fold (leaves) | Lee et al.2009a |
Overexpression of Fe transporter | Â | Â | Â | 1.1 fold (brown seeds) | Â |
 | OsActin1 pro-OsYSL15 | Japonica cv. Dongjin | Paddy field | 1.3 fold (brown seeds) | Lee et al. 2009b |
Approach 6 | 35S pro-OsIRO2 | Japonica cv. Tsukinohikari | Calcareous soil in greenhouse | 3 fold (brown seeds) | Ogo et al. 2011 |
Overexpression of transcription factor | Â | Â | Â | Â | Â |
Approach 7 | OsVIT1 or OsVIT2 T-DNA insertion mutant lines | Japonica cv. Zhonghua11 Japonica cv. Dongjin | Hydroponic culture | 1.4 fold (brown seeds) | Zhang et al. 2012 |
Knockdown of OsVITs genes | Â | Â | Â | Â | Â |
 |  |  | Paddy field | 1.4 fold (brown seeds) |  |
 | OsVIT2 T-DNA insertion mutant line | Japonica cv. Dongjin | Soil cultivation in greenhouse | 1.3 fold (brown seeds) | Bashir et al. 2013 |
 |  |  |  | 1.8 fold (polished seeds) |  |