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Table 1 Approaches of Fe biofortification of rice: single transgenic approaches

From: Iron biofortification of rice using different transgenic approaches

Approach Introduced genes Rice cultivar Cultivation condition Fold increase in Fe concentration compared to non-transgenic ricea References
Approach 1 OsGluB1 pro-SoyferH1 Japonica cv. Kitaake Soil cultivation in greenhouse 2 fold (polished seeds) Goto et al. 1999
Enhancement of Fe storage in rice seeds by ferritin      
     3 fold (brown seeds)  
  OsGluB1 pro- SoyferH1b Japonica cv. Kitaake Soil cultivation in greenhouse 1.5 fold (brown seeds) Qu et al. 2005
  OsGlb1 pro-SoyferH1b     
  OsGluB1 pro-SoyferH1 Japonica cv. Taipei 309 Soil cultivation in greenhouse 2.2 fold (brown seeds) Lucca et al. 2002
  OsGluB1 pro-SoyferH1 Indica cv. IR68144 Soil cultivation in greenhouse 3.7 fold (polished seeds) Vasconcelos et al. 2003
   (High Fe breeder line)    
  OsGluA2 pro-OsFer2 Indica cv. Pusa-Sugandh II (Aromatic rice) Soil cultivation in greenhouse 2.1 fold (polished seeds) Paul et al. 2012
Approach 2 OsActin1 pro-HvNAS1c Japonica cv. Tsukinohikari Soil cultivation in greenhouse 2 fold (polished seeds) Masuda et al. 2009
Enhancement of Fe translocation by overexpression of NAS      
  35S pro-HvNAS1c     
  Activation tag line of OsNAS3 Japonica cv. Dongjin Soil culture in greenhouse 3 fold (polished seeds) Lee et al. 2009c
  35S pro- OsNAS1, 2, 3 Japonica cv. Nipponbare Soil cultivation in greenhouse 4 fold (polished seeds) Johnson et al. 2011
Approach 3 OsSUT1 pro-OsYSL2 Japonica cv. Tsukinohikari Soil cultivation in greenhouse 4 fold (polished seeds) Ishimaru et al. 2010
Enhancement of Fe transportation by Fe transporter      
Approach 4 Barley IDS3 genome fragment Japonica cv. Tsukinohikari Andosol soil in paddy field 1.4 fold (polished seeds)
1.3 fold (brown seeds)
Masuda et al. 2008
Enhancement of Fe uptake and translocation by IDS3 gene      
    Calcareous soil in paddy field 1.3 fold (brown seeds) Suzuki et al. 2008
Approach 5 Ubiquitin pro-OsIRT1 Japonica cv. Dongjin Paddy field 1.7 fold (leaves) Lee et al.2009a
Overexpression of Fe transporter     1.1 fold (brown seeds)  
  OsActin1 pro-OsYSL15 Japonica cv. Dongjin Paddy field 1.3 fold (brown seeds) Lee et al. 2009b
Approach 6 35S pro-OsIRO2 Japonica cv. Tsukinohikari Calcareous soil in greenhouse 3 fold (brown seeds) Ogo et al. 2011
Overexpression of transcription factor      
Approach 7 OsVIT1 or OsVIT2
T-DNA insertion
mutant lines
Japonica cv. Zhonghua11 Japonica cv. Dongjin Hydroponic culture 1.4 fold (brown seeds) Zhang et al. 2012
Knockdown of OsVITs genes      
    Paddy field 1.4 fold (brown seeds)  
  OsVIT2 T-DNA
insertion mutant line
Japonica cv. Dongjin Soil cultivation in greenhouse 1.3 fold (brown seeds) Bashir et al. 2013
     1.8 fold (polished seeds)  
  1. aThe tissue name written in Parentheses is the rice tissue where Fe concentration was increased. bThey introduced these two genes into same transgenic lines. c These two genes were introduced separately into rice and they analyzed these two types of transgenic lines.