Localization of flo7 (t) on the linkage map of rice chromosome 5 (A), and e-Landings of significant SSR markers on a reference rice genome to delimit the corresponding 1.04 Mbp virtual contig (B). SSR markers were applied based on F-statistics from single-locus ANOVA results to narrow down the putative location responsible for the floury endosperm characteristics (see Table 4; ANOVA). The accumulative genetic distances shown in centiMorgans (cM) were calculated using the observed recombination fractions between SSR marker pairs in the F2 mapping population based on the Kosambi mapping function (see also Table 4; Mirror-map information). Note that the SSR markers, placed at 97.8 cM (RM164, RM18620, RM18624) and 101.6 cM (RM18648, RM1386) were co-segregated each other in the F2 mapping population from Suweon 542/Milyang 23 (N = 94). The 1.04 Mbp virtual contig, composed of overlapping 10 BAC/PAC clones, was delimited by e-Landings of six significant SSR markers on the reference rice genome, ‘Os-Nipponbare-Reference-IRGSP-1.0’. The underlined clone (OJ1174_H11) includes the genomic sequences corresponding to RM18639, the most significant SSR marker for the variation in FGP, and the OsPPDKB gene (Kang et al.2005).