Skip to main content
Rice
Figure 3 | Rice

Figure 3

From: Involvement of mitogen activated protein kinase kinase 6 in UV induced transcripts accumulation of genes in phytoalexin biosynthesis in rice

Figure 3

Staurosporin and MAPK cascade specific inhibitors attenuate UV induced kinase activity and PA gene expression. Rice plants were pre-treated with inhibitors and irradiated with UV. As a control one set of plants were pre-treated with 0.1% DMSO and irradiated with UV or left unirradiated. a Effect of staurosporin on UV induced MAPK activity. b Effect of U0126, PD169316 and SB202190 on UV induced MAPK activity. Kinase activity was assayed by performing in-solution kinase assay using MBP as substrate. c Effect of staurosporin, U0126, PD169316 and SB202190 on UV induced transcripts accumulation of genes of PA biosynthesis. Transcripts accumulation of OsKSL4, CYP99A3 and OsMAS was studied by RT-PCR. Expression of rice actin gene used as a loading control.

Back to article page