Characterization of the OsVIT2 knockdown mutant ( Osvit2 ) and expression of OsVIT2 during different developmental stages of rice. a: Gene structure and positions of the primers specific for the T-DNA insertion. b: Bands corresponding to the primers are shown in red. c: Bands corresponding to the primers are shown black. d: Quantitative reverse-transcription PCR analysis showing the expression of Osvit2 in WT and knockdown plants. A vertical bar followed by an asterisk indicates a significant difference from WT according to the Tukey–Kramer test (p < 0.05; WT, n = 4; osvit2, n = 8). e: Roots at 27 and 76 DAT. f: Stems at 27 and 76 DAT. g: Leaf blades at 27, 76, and 125 DAT. h: The anther size was 0.3–0.6, 0.7–1.0, 1.2–1.5, and 1.6–2.0 mm. i: Embryos 1, 3, 5, and 7 days after flowering. j: Ovaries 7, 10, 14, 28, and 42 days after flowering. k: Endosperm, 7, 10, 14, 28, and 42 days after flowering. l: The pistil size was 5–10, 10–14, and 14–18 mm (n = 3). Error bars represent the SD.