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Figure 4 | Rice

Figure 4

From: Isolation of a novel mutant gene for soil-surface rooting in rice (Oryza sativa L.)

Figure 4

Map position and complementation of the candidate gene for soil-surface rooting. (A) Fine mapping of the SOR1 locus, carried out by using SOR-type plants, indicated that the DNA marker RM551 on chromosome 4 co-segregated with soil-surface rooting. The SOR1 locus was identified in a 136-kb region between markers RM16254 and 2935–6. The Os04g0101800 gene is shown as a black arrow, with white boxes indicating the exons. The second exon of the sor1 mutant allele contains a 33-bp genomic deletion. FW and RV, specific primers used to confirm the deletion. (B) Alignment of SOR1 (wild-type and mutant) amino acid sequences. The positions of the zinc finger (RING finger) domain (aa124–170) and the vWFA (von Willebrand factor type A) domain (aa279–460) are indicated above the sequences. The 11-aa deletion (aa332–342) in the vWFA domain of the mutant is shown with dashes (-). (C) Confirmation of the genomic deletion in the mutant by analysis of the PCR amplicon size in wild-type (WT), mutant (MT), and transgenic plants (Tg1–3). Tg1 and Tg2 were transformed with a6677-bp genomic fragment containing Os04g0101800; Tg3 was transformed with an empty vector. M, DNA marker. (D) Phenotyping of soil-surface rooting by the glass tube method (top line) and ratio of soil-surface rooting (SOR) by the basket method (bottom line) in the mutant, wild-type, and transgenic plants.

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