Figure 3
From: Isolation of a novel mutant gene for soil-surface rooting in rice (Oryza sativa L.)
Gravitropic response assay for shoots and primary roots at the seedling stage. A diagram illustrating the assay is shown in A–C. Germinated seeds are sown just under the surface of 1.5% agar in plastic trays with the embryo placed upward (A). Trays were set on their short sides 18 h after sowing (B) and rotated 90° clockwise 10 h later (C). Differences in root gravitropic responses in mutant and Nipponbare seedlings 10 h after setting the tray on a short edge (D), 10 h after rotation (E), and 76 h after rotation (F). Black dots in D–F mark the location of seminal root tips just before the rotation. Scale bars, 2 cm. The direction of gravity (G) is indicated by arrows.