Proteomic analysis of a disease-resistance-enhanced lesion mimic mutant spotted leaf 5 in rice

Rice

Table 2 Gene-specific primers for RT-PCR in this study

^aSpot id	^bAccession	Forward primer (5’-3’)	Reverse primer (5’-3’)	^c Tm (°C)
1	LOC_Os12g37540	AACAAGGTAGGGATAGTTACTT	CCTTGTATGTGGGTTTTTTAGAA	55
2	AK067692	AATGAAATCTTGCTTGCTGC	CTAAATCTTCTTGGGACATA	60
3	AK067692	GCCTACTTCTTCACATTCAC	ATTTCATTACCTTCACGAGC	55
4	AK067732	ACCCGCTTTATTCTGCTG	ATCCTTTTGACACAGTATGG	60
5	AK104875	AGATGCTGTGCTTGATGCCT	CAATGACGAAGCGACCAGAT	55
6	AK105059	CAAACAGGGTGAAGAGCCAG	CTCGCATTTAGCCAGGGACA	62
7	AK065872	ACGCCGACAAGAATCCCAAC	CAATACGACCAGCCCCAACA	60
8	AK064960	TTCATCACCACCGACTACAT	AACCCTCAACAATACCAAAC	55
9	AK060847	GAAGCTGAAGAAGCAGGTGACATC	CGAAGACGAGCTCACACTGGAAG	55
10	AK104332	ATGGGTGAATTCTGTGGTGAG	CCCTTCTTGATGATGTCTGCC	58
11	AK102889	CTCGTTGCGGTAGTGCTGCT	AATGAAATCTTGCTTGCTGC	55
12	AK099598	TGGCAGCGAAGACAAACAAC	CTGGAAGAGCACCGACGAAA	62
13	AK063934	GCTTGAGATTTGATGTTGAG	GTCCTCTGCGTATTTTTCTG	62
14	AK070067	CGACTTCTCCACCCTACTAT	ATGATGTTGGTGATGACGCC	55

^a Spot identity of protein in 2-D gel image; ^bmRMA accession of the corresponding protein in GenBank (http://www.ncbi.nlm.nih.gov/), except for spot 1 protein whose gene accession was only deposited in TIGR database (http://rice.plantbiology.msu.edu/index.shtml); ^cAnneal temperature of primer pair used in RT-PCR.