Differential gene expression in gall midge susceptible rice genotypes revealed by suppressive subtraction hybridization (SSH) cDNA libraries and microarray analysis

Rawat, Nidhi; Neeraja, Chiruvuri Naga; Nair, Suresh; Bentur, Jagadish S

doi:10.1186/1939-8433-5-8

Rice

Table 1 Primer sequence information for 20 selected genes chosen for real-time PCR assay

From: Differential gene expression in gall midge susceptible rice genotypes revealed by suppressive subtraction hybridization (SSH) cDNA libraries and microarray analysis

S.No	Gene Name	Locus ID	Primer sequences	Tm (°C)	Product size (bp)
1	geranylgeranyl pyrophosphate synthetase	loc_os02g44780	F: GACAGGTTCGGTGACAAGTATGG R: CGCATAGTCCACGAAGCTGTAC	60	63
2	cycloartenol-C-24-methyltransferase 1	loc_os07g10600	F: TGGATCCAAGCCGGTTTTC R: AATTGCACGCCCCACAGTA	59	59
3	outer mitochondrial membrane protein porin	loc_os03g10510	F: CCAAGTACAGTGCTGCGATAGG R: GCCAGAACGATTGCAGCAT	58	61
4	peptide transporter PTR2	loc_os05g33960	F: TACTACCTCGTCTGCTTCCACTTCT R: TCACACTCCTTCTCGTCATCGT	59	80
5	fructose-bisphosphate aldolase	loc_os01g67860	F: CCATCGCAGCTTTCCATTG R: TCCTGGCAGATGATGGCATA	60	65
6	glyceraldehyde-3-phosphate dehydrogenase	loc_os06g45590	F: CAAGGCTGGAATTGGCTTAAGTT R: GTAGCCCCACTCGTTGTCGTA	58	70
7	pyruvate dehydrogenase E1 component	loc_os06g13720	F: ATCGAAAAGCCCCGCATACT R: TGACTGCAAGAACATCCATACCA	59	80
8	catalase-1	loc_os03g03910	F: TCAACCGCAACATCGACAAC R: TCCCCGGCACGATGATC	62	75
9	DELLA protein RGL1	loc_os03g48450	F: ACTGTGCCACGGGATGATG R: CTTCCCGGCCAAATAGATCTC	60	61
10	NADH oxidase	loc_os01g53294	F: GAAAGAGGAAAAGCCGAAAAGG R: CCCACCGGATTACCGAAAC	59	69
11	Atrboh F	loc_os05g38980	F: TGGGTCTCCAACACTTACGAAA R: TCGTTGTCGTCTGGCTGAATT	58	65
12	NBS-LRR	loc_os02g55550	F: ACTATGCTCCCGGTTCCCTAA R:ATCGGTCAGTGAAGAGCAGTGA	58	130
13	NAC domain containing protein	loc_os08g10080	F: AAGGAGGACTGGGTGCTATGC R: TCTTCAGATGATGGGCTTGGA	58	71
14	TCTP	loc_os11g43900	F: GATGGCGGCTTGGTGTTT R: CAGCCCATGAGAGAAGTAAAGGA	60	75
15	Chitin inducible gibberallin	loc_os07g36170	F: CCAATCCAACGTCTAGGTGCTT R: TTTGTGCCAGAGTTTCCATGTCTA	58	68
16	Substrate Transporter	loc_os06g02370	F: GCAGCTTCCTCGGCATCAT R: CTTCAGCGCCCCGATGT	59	62
17	ABC transporter	loc_os04g38570	F: CGAATGCAATGGAGAGGAAGAC R: ACCGCATATAACCCAGTTCCAA	57	67
18	CBS domain containing protein	loc_os03g52690	F: TATCGGGTCACATGGCAGTCT	58	70
			R: AATAGATGGTTTTGGAGCACTGATG
19	Lipase	loc_os09g22450	F: AAGCAAAGCATGATGCAAGAGAT R: GCTCAGTTCAAGGCCTCCAA	58	111
20	OsMADS18 - MADS-box family gene	loc_os07g41370	F:CGGGAGGAGCAAAATGGA R:GAGCCGTCACTGGTGTTGGT	60	58

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