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Figure 4 | Rice

Figure 4

From: Narrowing down the targets for yield improvement in rice under normal and abiotic stress conditions via expression profiling of yield-related genes

Figure 4

qRT-PCR confirms altered expression of few yield-related genes under abiotic stress conditions. Histograms (A-H) depict fold change (log2 scale) in expression of stress-regulated yield-related genes viz. (A) D3, (B) LRK1, (C) OsEATB, (D) RCN1, (E) OsSPL14, (F) LOG, (G) DEP1, (H) SP1; under different abiotic stress conditions – cold, drought, salinity, and heat as obtained via qRT-PCR. For expression analysis by qRT-PCR, 10 day old seedlings of IR64 variety (a moderately sensitive cultivar) of rice were subjected to stress treatment for 6 hours followed by RNA isolation, first strand cDNA synthesis and real-time PCR. Error bars show standard deviation. (I) Heat map generated on the basis of above changes in gene expression using average linkage hierarchical clustering with Pearson correlation as the distance metric. Color bar represents scale for log2 fold change in expression.

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