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Figure 8 | Rice

Figure 8

From: OsRap2.6 transcription factor contributes to rice innate immunity through its interaction with Receptor for Activated Kinase-C 1 (RACK1)

Figure 8

OsRap2.6 Ox plants are resistant to a compatible race (007) of M. oryzae. OsRap2.6 mRNA transcript levels from three independent Ox plants (P1, P6 and P14) were measured by qPCR. The Ox plants were grown in the greenhouse for two months and inoculated with M. oryzae compatible (virulent) Ina 86–137 (Race 007) fungal spore suspension. (A) Expression levels of OsRap2.6 transcripts in T1 OsRap2.6 Ox plants before infection with rice blast fungus. (B) Photographs showing lesions in leaf blades in WT and OsRap2.6 Ox plants after infection. (C) Quantitative analysis of fungal growth showing increased resistance in OsRap2.6 Ox plants 7 days after infection with rice blast fungus compatible race (007). Ubiquitin was used as an internal control. Bars represent the means ± SE calculated using four biological replicates where each replicate consists of three independent technical replicates (p ≤ 0.01, n=48). (D) Lesion length of OsRap2.6 Ox plants showing increased resistance to blast fungus as compared to WT as shown by increased lesion length after infection (p ≤ 0.01, n=48). (E) Expression of PAL1 mRNA in OsRap2.6 Ox plants after infection with the compatible race 007. Levels of PAL1 mRNA were up regulated as measured by reverse transcription qPCR (p ≤ 0.01, n=48).

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