Molecular mapping of the Pi2/9 allelic gene Pi2-2 conferring broad-spectrum resistance to Magnaporthe oryzae in the rice cultivar Jefferson

Rice

Table 1 Polymorphic SSR markers around the Pi2/9 locus used for linkage analysis

Markers	Forward primer (5′-3′)	Reverse primer (5′-3′)	Genomic position (bp)	Expected size (bp)
MRG5836^a	AAAAACCTAGAAAATGGGAAAATG	TATAAGCCGCAGCCAAATTC	9308979-9309076	98
RM19817^b	CCAAGGAGGTGATCCAGGAGTGC	CGGCAGAGCAGACGACATGG	10137012-10137394	383
RM7178^b	CCGTGAGATGGGCTACCTAC	TAACCTTCACAGCGAACGTG	10198893-10199043	151
AP5659-5^a	CTCCTTCAGCTGCTCCTC	TGATGACTTCCAAACGGTAG	10357166-10357453	288
AP5659-3^a	TCTTTCCTAGGGAACCAAAG	AAGTAGTTGCTGAGCCATTG	10406597-10406825	229
RM7311^b	CGTGGCGCCTTTAATCTC	AGTGGTCGTTGAACTCGGAG	11045702-11045848	147

^a Previously reported markers in this region.
^b SSR markers released by Gramene database (http://www.gramene.org/db/markers/).
Genomic position and expected PCR product size for each marker were determined based on the reference sequence of rice cultivar Nipponbare released by International Rice Genome Sequencing Program (IRGSP).