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Rice
Figure 5 | Rice

Figure 5

From: Rice UBC13, a candidate housekeeping gene, is required for K63-linked polyubiquitination and tolerance to DNA damage

Figure 5

Quantitative analyses of OsUBC13 expression. a Expression of OsUBC13 (LOC_Os01g48280) during rice development. Samples were taken from different developmental stages as indicated and relative transcript levels of the entire transcriptome were determined by microarray analysis. The data is retrieved from Genevestigator (www.genevestigator.com). b Relative OsUbc13 protein level in rice seedlings after treatment with various stresses, two weeks after germination rice seedlings were treated with the given biotic or abiotic agent for the predetermined time as indicated on top of each panel and total proteins were extracted from the whole plants. The cellular OsUbc13 was detected by western bolt analysis using the anti-hUbc13 mAb 4E11 (Andersen et al. [2005]). Each lane contains 20 μg total protein and the most abundant protein band as visualized by Coomassie brilliant blue staining serves as a loading control (the bottom image of each panel). CK, untreated seedlings; Ct, cisplatin.

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